sophorose) linked by a glycosidic bond through a hydroxyl group located at the penultimate position of an 18-carbon fatty acid. This type of biosurfactant occurs as a mixture of macrolactone and open-chain (free acid) forms

Sophorolipids (SLs) production by the yeast Candida bombicola was studied in batch shake flasks using synthetic dairy wastewaters (SDWW) with or without any added external carbon and nitrogen sources. A maximum SLs production of 38.76 g/l was observed with the SDWW supplemented with low cost substrate of sugarcane molasses at 50 g/l and soybean oil at 50 g/l. When the SDWW was supplemented with more costly glucose, yeast extract, urea and soybean oil, the production, however, got lowered to only 29.49 g/l, but with a maximum biomass production of 17.38 g/l together with a complete utilization of the carbon sources. Keywords—Candida bombicola, dairy wastewater, fat and oil, sophorolipids.